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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really however out there.

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Blocking peptides are peptides that bind exclusively into the focus on antibody and block antibody binding. These peptide normally consists of the epitope regarded via the antibody.

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This guidebook outlines even further feasible optimization of cellular and immunoassay parameters to be sure the absolute best results are...

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This information outlines further more possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...

Antibodies certain to the blocking peptide not bind to the epitope around the goal protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is distinct.

Concentrate on Info Cyclin E belongs for the really conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance in the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which lead to the temporal coordination of every mitotic event. Cyclin E varieties a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary and is particularly degraded as cells development through S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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